Monday, September 27, 2010

How do hematology analyzers work?

How does it detect WBC, RBC, hemoglobin, platelets. What is the process? There are two chambers. Each uses flow cytometry, substance the cells are crumpled up and pushed along tubing passed a light source. The buoyant is picked up on a detector. When a cell passes by, the neutral flow is interrupted and it causes a "blip" within the detector. The size of the "blip" is proportional to the size of the cell.
In one chamber, the red cells are counted. The detecter is set to count merely the cells that decline with surrounded by the size limits of Red Blood Cells.
In the other chamber, the blood is put into a solution that lyses the red blood cell leaving merely WBC and platelets intact. The detector is set to count cells that plummet in the platelet catalogue and in the WBC scale of sizes, nuclei size and shape is also detected here and is used to differentiate one type of WBC from another. It also quantify hemoglobin here with spectrophotometry.
view bld thru mircoscope and with experience in histology of bld and its compositions, they r competent to differenciate b/t the cells fnd contained by bld. they no that rbcs r most numerous and the the they dont have nucleus. platelets r muc smaller in size relative to rbc. wbc r bigger than the rbc after they r competent to count them just taking a small nouns of the view and near this compare it to the values that r supposed to b fnd in the mundane human and give a possible diagnosis for the medical doctor to confirm

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